Giemsa stain

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  • Giemsa stain is used to distinguish all three types in blood smears.
  • He is remembered for creating a dye solution commonly known as "Giemsa stain".
  • It was first discovered by using the Giemsa staining method on one chromatid belonging to the sister chromatid complex before anaphase in mitosis.
  • Giemsa stain is a classic blood film stain for peripheral blood smears and bone marrow specimens.
  • The Giemsa staining was able to stain due to the presence of bromodeoxyuridine analogous base which was introduced to the desired chromatid.
  • These samples can be stained with the Giemsa stain.
  • Although largely superseded by techniques like Giemsa staining, the Gimenez technique may be valuable for detecting certain slow-growing or fastidious bacteria.
  • Routine analysis of blood in medical laboratories is usually performed on blood films stained with Romanowsky, Wright's, or Giemsa stain.
  • Tissue biopsy and Wright-Giemsa stain are used to aid in the diagnosis.
  • The preferable method for diagnosis of P. malariae is through the examination of peripheral blood films stained with Giemsa stain.
  • Giemsa stain is used in Giemsa banding, commonly called G-banding, to stain chromosomes and often used to create an idiogram.
  • Giemsa stain is also used to visualize chromosomes.
  • Pappenheimer bodies are visible with a Wright and/or Giemsa stain.
  • Giemsa staining may aid in identifying the flagellate.
  • Left smear is unstained, right smear is stained with Wright-Giemsa stain.
  • Giemsa stain is also a differential stain, such as when it is combined with Wright stain to form Wright-Giemsa stain.
  • Giemsa staining will uniquely stain B. malayi sheath pink.
  • Also, the sheath of B. timori does not stain pink with Giemsa stain as is observed with B. malayi and W. bancrofti.
  • To definitively diagnose the disease, C. ruminantium must be demonstrated either in preparations of the hippocampus under Giemsa staining or by histopathology of brain or kidney.
  • It is worth noting that the widespread contamination with HeLa cells was initially recognized by Walter Nelson-Rees using simple Giemsa stain karyotyping under a light microscope.
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