All Noun
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  • Several other enzymes that seem to be involved in deadenylation have been identified in yeast.
  • The rate of deadenylation may also be regulated by RNA-binding proteins.
  • The demonstration that hCUGBP1 is involved in the control of mRNA deadenylation and instability like xCUGBP1 came next.
  • MicroRNAs that are partially complementary to a target can also speed up deadenylation, causing mRNAs to be degraded sooner.
  • The first human mRNA reported to be targeted to rapid deadenylation and degradation by CUGBP1 is the oncogene c-jun.
  • Because deadenylation is often the rate limiting step of mRNA degradation the enhancement of deadenylation increases mRNA turnover.
  • Binding of the miRNA can hinder translation of mRNA by promoting degradation or inducing deadenylation.
  • Alterations of the enzyme due to adenylation and deadenylation.
  • Interestingly, such a motif is found in a number of unstable mRNAs in human cells suggesting that they are degraded by a CUGBP1 deadenylation dependant pathway.
  • This deadenylation and degradation process can be accelerated by microRNAs complementary to the 3' untranslated region of an mRNA.
  • Similarly, in Xenopus, the miR-430 ortholog miR-427 has been shown to target maternal mRNAs for deadenylation.
  • Years ago, it was shown that the class III ARE (devoid of any AUUUA motif) of the human c-jun oncogene directed rapid deadenylation and degradation to a reporter mRNA.
  • In zebrafish, the microRNA miR-430 is expressed at the onset of zygotic transcription and targets several hundred mRNAs for deadenylation and degradation.
  • The other pathway by which mRNA is degraded is by deadenylation from 3'-5'.
  • The binding of CUGBP1 to the 3'UTR of mRNAs bearing GU-rich element would target these mRNAs for rapid deadenylation by PARN and subsequent degradation.
  • In mammalian cell extract as well as in xenopus egg extracts, depletion and rescue experiments showed that specific binding of CUGBP1 to the 3'UTR of mRNA is required for the targeted specific deadenylation to occur.
  • Recent work on miR-430 in zebrafish, as well as on bantam-miRNA and miR-9 in Drosophila cultured cells, shows that translational repression is caused by the disruption of translation initiation, independent of mRNA deadenylation.
  • The encoded protein recognizes and binds to a constitutive decay element (CDE) in the 3' UTR of mRNAs, leading to mRNA deadenylation and degradation.
  • Finally, with the miR-155-5p/-3p acting as an adaptor for the RISC, complex-bound mRNAs are subjected to translational repression (i.e. inhibition of translation initiation) and/or degradation following deadenylation.